Volume: 45 Issue: 4
Phytochemical estimation and antimicrobial activity assessment of different solvent extracts of Tridax procumbens L.
Year: 2023, Page: 123-132, Doi: https://doi.org/10.62029/jmaps.v45i4.joshi
Received: Dec. 1, 2023 Accepted: Dec. 12, 2023 Published: Dec. 31, 2023
This study estimates the pharmacological properties of T. procumbens using qualitative and quantitative analysis of secondary metabolites, antioxidants, and antimicrobial activity analysis. Using standard methods, four aqueous extracts were prepared and screened for alkaloids, flavonoids, saponins, tannins, phenol, and ascorbic acid. The antioxidant profiling was undertaken with the support of 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical, ferric reducing antioxidant power (FRAP) and reducing power assay. The antimicrobial assay of crude fractions was conducted against human pathogens E. coli (MTCC 443) and Aspergillus niger (ITCC4718) using MIC analysis. Catalase (CAT), peroxidase (POX), superoxide dismutase (SOD) and ascorbate peroxidase (APX) activity was performed following standard methods for correlating antimicrobial properties with reactive oxygen species (ROS) scavenging activity. Tridax procumbens contains adequate amounts of carbohydrates and protein with some secondary metabolites. The extract also showed the presence of a significant quantity of antioxidants and satisfactory antimicrobial activity.
Keywords: Antimicrobial activity, Enzymatic antioxidants, Phytochemicals, Solvent extracts, Tridax procumbens
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© CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015
Joshi A, Patra PK, Mishra PK, Khodiar PK, Sahu GK.2023. Phytochemical estimation and antimicrobial activity assessment of different solvent extracts of Tridax procumbens L. J Med Aromat Plant Sci 45: 123-132.
