Volume: 45 Issue: 4
Effect of drying techniques on essential oil yield and quality of lemongrass [Cymbopogon flexuosus (Nees ex Steud) Wats] cultivars Krishna and CKP-25
Year: 2023, Page: 143-151, Doi: https://doi.org/10.62029/jmaps.v45i4.devi
Received: Dec. 1, 2023 Accepted: Dec. 18, 2023 Published: Dec. 31, 2023
An experiment was conducted on popularly cultivated two cultivars (cv.Krishna and cv. CKP-25) of Lemongrass [C. flexuous (Nees ex Steud) Wats] at FFDC, Kannauj in August 2015 and replicated in February 2016 to examine the effect on different drying techniques by adopting other parameters. The study recorded different parameters at intervals of 7 days during plantation. Cultivar Krishna was more favourable in herbage yield, oil percentage, and oil yield than cv. CKP-25.The semi-dried condition of CV. Krishna was found to be highest in oil percentage, i.e., 1.5 ± 0.08%, followed by shade and sun-shade-dried oil percentage was found as 1.4 ± 0.07% each, and the most negligible value was by fresh leaves, i.e., 0.7 ± 0.03%, whereas cv. CKP-25 oil percentages in semi-dried and shade-dried were found to be highest at 0.9 ± 0.05% each; sun-shade-dried and fresh leaves were 0.8 ± 0.04% and 0.6 ± 0.03%, respectively. In physical analysis, the value of cv. Krishna showed optical rotation in the ranges of -3.0 to -1.62, a refractive index of 1.4785 to 1.4791 at 27 °C, and a specific gravity of 0.8901- 0.8932 at 27°C. However, cv. CKP-25 showed optical rotation in the ranges of -2.4 to -1.31, a refractive index of 1.4724- 1.4829 at 27 °C, and a Specific gravity of 0.8819- 0.8854. All the values were under the range of BIS standards. In an instrumental analysis of the GLC report, citral content was recorded cv. CKP-25 showed a higher citral content of 83.95% in semi-dried conditions than cv. Krishna of 74.5%. The cv. (Krishna) in sun-shade dried, had 79.88%, followed by shade-dried at 76.83% and fresh leaves at 75.71%. Whereas, in cv. CKP-25, sun-shade-dried 83.24%, shade-dried 82.19%, and freshly harvested leaves showed 76.31% citral content, respectively.
Keywords: Citral content, CKP-25, Cymbopogon flexuosus cv. Krishna, Drying methods, Lemongrass, Oil percentage, Physical analysis
Avoseh, O., Oyedeji, O., Rungqu, P., Nkeh-Chungag, B. N., & Oyedeji, A. S. (2015). Cymbopogon species: Ethnopharmacology, phytochemistry and the pharmacological importance. Molecules, 20(5), 7438–7453.
Barisić, L., Cakić, M., Mahmoud, K. A., Liu, Y. N., Kraatz, H. B., Pritzkow, H., & Rapić, V. (2006). Helically chiral ferrocene peptides containing 1′-aminoferrocene-1-carboxylic acid subunits as turn inducers. Chemistry (Weinheim an der Bergstrasse, Germany), 12, 4965–4980.
Bartley, J. P., & Jacobs, A. L. (2000). Effects of drying on flavour compounds in Australian-grown ginger (Zingiber officinale). Journal of the Science of Food and Agriculture, 80, 209–215.
Bharti, S. K., Kumar, A., & Prakash, O. (2013). Essential oil of Cymbopogon citratus against diabetes: Validation by in vivo experiments and computational studies. Journal of Bioanalysis & Biomedicine, 5, 194–203.
Caputo, L., Amato, G., de Bartolomeis, P., De Martino, L., Manna, F., Nazzaro, F., & Barba, A. A. (2022). Impact of drying methods on the yield and chemistry of Origanum vulgare L. essential oil. Scientific Reports, 12, 3845.
Caputo, L., Amato, G., de Bartolomeis, P., De Martino, L., Manna, F., Nazzaro, F., De Feo, V., & Barba, A. A. (2022). Impact of drying methods on the yield and chemistry of Origanum vulgare L. essential oil. Scientific Reports, 12, 3845. https://doi.org/10.1038/s41598-022-07841-w. PMID: 35264638; PMCID: PMC8907181.
Chandra, K. S. J., & Narayanan, K. N. (1971). Studies on the supernumery chromosome in the genus Cymbopogon. In First All India Congress of Cytology and Genetics, 104–112.
Chauhan, N. K., Semwal, M. P., Singh, D., Singh, B., & Rauthan, S. (2017). Influence of various plant spacing on growth, herbage yield, essential oil yield and aroma content of palmarosa (Cymbopogon martini Roxb.) at different harvest under agroclimatic condition of Doon Valley. Journal of Essential Oil Bearing Plants, 20, 1587–1593.
Consueledo Díaz-Maroto, M., Pérez-Coello, M. S., & Cabezudo, M. D. (2002). Effect of drying method on the volatiles in bay leaf (Laurus nobilis L.). Journal of Agricultural and Food Chemistry, 50, 4520–4524. https://doi.org/10.1021/jf011573d. PMID: 12137470.
Devi, M. A., Nameirakpam, B., Devi, T. B., Mayanglambam, S., Singh, K. D., & Sougrakpam, S. (2020). Chemical compositions and insecticidal efficacies of four aromatic essential oils on rice weevil Sitophilus oryzae L. International Journal of Tropical Insect Science, 40, 549–559. https://doi.org/10.1007/s42690-020-00102-1.
Devi, M. A., Sahoo, D., Singh, T. B., & Rajashekar, Y. (2019). Toxicity, repellency and chemical composition of essential oils from Cymbopogon species against red flour beetle Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). Journal of Crop Protection and Food Safety, 15, 181–191. https://doi.org/10.1007/s00003-019-01264-y.
Devi, M. A., Sahoo, D., Singh, T. B., & Rajashekar, Y. (2021). Antifungal activity and volatile organic compounds analysis of essential oils from Cymbopogon species using solid-phase micro-extraction–gas chromatography–mass spectrometry. Journal of Agricultural and Food Research, 3, 100110. https://doi.org/10.1016/j.jafr.2021.100110.
Elhag, B. E. M. (2018). Production of lemongrass (Cymbopogon flexuosus L.) and evaluation of its leaf essential oil efficacy against some plant pathogens, Gezira State, Sudan (Doctoral dissertation, University of Gezira).
Figiel, A., Szumny, A., Gutierrez-Ortiz, A., & Carbonell-Barrachina, A. (2010). Composition of oregano essential oil (Origanum vulgare) as affected by drying method. Journal of Food Engineering, 98, 240–247. https://doi.org/10.1016/j.jfoodeng.2010.01.002.
Frankel, E. N., Huang, S. W., & German, J. B. (1994). Interfacial phenomena in the evaluation of antioxidants: Bulk oils vs emulsions. Journal of Agricultural and Food Chemistry, 42, 1054–1059.
Ganjewala, D., & Gupta, A. K. (2013). Lemongrass (Cymbopogon flexuosus Steud.) Wats essential oil: Overview and biological activities. Recent Progress in Medicinal Plants, 37, 235–271.
Ganjewala, D., Kumari, A., & Khan, K. H. (2008). Ontogenic and developmental changes in essential oil content and compositions in Cymbopogon flexuosus cultivars. International Journal of Essential Oil Research, 3, 56–65.
Gupta, B. K. (1969). Studies in the genus Cymbopogon Spreng: II. Chemocytotaxonomic studies in Indian Cymbopogons. In Proceedings of the Indian Academy of Sciences, 70, 241–247.
Hanaa, A. M., Sallam, Y. I., El-Leithy, A. S., & Aly, S. E. (2012). Lemongrass (Cymbopogon citratus) essential oil as affected by drying methods. Annals of Agricultural Sciences, 57, 113–116. https://doi.org/10.1016/j.aoas.2012.08.004.
Hassan, E. (2016). Comparative study on the biosorption of Pb(II), Cd(II) and Zn(II) using lemongrass (Cymbopogon citratus): Kinetics, isotherms and thermodynamics. Chemistry International, 2, 89–102.
Jayasingha, P. (1999). Lemongrass: A literature survey (Medicinal and Aromatic Plant Series No. 09).
Kassahun, B. M., Mekonnen, S. A., Abedena, Z. T., Kidanemariam, H. G., Yalemtesfa, B., Atnafu, G., & da Silva, J. A. T. (2011). Performance of lemongrass (Cymbopogon citratus L. (DC) Stapf) agronomic and chemical traits in different agro-ecologies of Ethiopia. Medicinal and Aromatic Plant Science and Biotechnology, 5, 133–138.
Katiyar, S. K. (2011). Green tea prevents non-melanoma skin cancer by enhancing DNA repair. Archives of Biochemistry and Biophysics, 508, 152–158.
Khanuja, S. P. S., Shasany, A. K., Pawar, A., Lal, R. K., Darokar, M. P., Naqvi, A. A., & Kumar, S. (2005). Essential oil constituents and RAPD markers to establish species relationship in Cymbopogon Spreng (Poaceae). Biochemical Systematics and Ecology, 33, 171–186.
Kumar, A., Jnanesha, A. C., Chanotiya, C. S., & Lal, R. K. (2023). Climate-smart lemongrass (Cymbopogon khasianus (Hack.) Stapf ex Bor) yields quality essential oils consistently across cuttings and years in semi-arid, tropical Southern India. Biochemical Systematics and Ecology, 110, 104716.
Kumari, R., Agrawal, S. B., & Sarkar, A. (2009). Evaluation of changes in oil cells and composition of essential oil in lemongrass (Cymbopogon citratus (DC) Stapf.) due to supplemental ultraviolet-B irradiation. Current Science, 1137–1142.
Kuriakose, K. P. (1995). Genetic variability in East Indian lemongrass (Cymbopogon flexuosus Stapf). Indian Perfumery, 39, 76–83.
Lal, M., Bora, A., & Pandey, S. K. (2019). Identification of a new high geraniol rich variety “Jor Lab L-15” of lemongrass [Cymbopogon khasianus (Hack) Stapf (ex Bor)]. Journal of Essential Oil Bearing Plants, 22, 972–978.
Lal, M., Dutta, S., & Bhattacharyya, P. R. (2016a). Development of a high yielding variety, Jor Lab L-8 of lemongrass (Cymbopogon flexuosus L.). Annals of Agricultural and Biological Research, 21, 22–23.
Lal, M., Dutta, S., & Bhattacharyya, P. R. (2016b). Development of a new superior variety (Jor Lab C-5) of Java citronella with characteristics of stable and high oil yield. Annals of Biology, 32, 22–23.
Lal, M., Dutta, S., Munda, S., & Pandey, S. K. (2018). Novel high value elemicin-rich germplasm of lemongrass (Cymbopogon khasianus (Hack) Stapf (ex Bor)) from North East India. Industrial Crops and Products, 115, 98–103.
Lawrence, B. Á. (1985). A review of the world production of essential oils (1984). Perfumer & Flavorist, 10, 1–16.
Lawrence, B. M. (2004). Aromatic plants for the flavor and fragrance industries. In Encyclopedia of Plant and Crop Science. CRC Press.
Mehrotra, R., Lothe, N. B., & Verma, R. K. (2023). Comparative study on changes in biochemical constituents, yield and quality of lemongrass (Cymbopogon flexuosus) grown under different soil types. Archives of Agronomy and Soil Science, 1–17.
Mirjalili, M. H., Salehi, P., Vala, M. M., & Ghorbanpour, M. (2019). The effect of drying methods on yield and chemical constituents of the essential oil in Lavandula angustifolia Mill. (Lamiaceae). Indian Journal of Plant Physiology, 24. https://doi.org/10.1007/s40502-019-0438-4.
Molangui, T., Menut, C., Bouchet, P., Bessière, J. M., & Habimana, J. B. (1997). Aromatic plants of tropical Central Africa. Part XXX. Studies on volatile leaf oils of 10 species of Eucalyptus naturalized in Rwanda. Flavour and Fragrance Journal, 12, 433–437.
Munda, S., Begum, T., Gogoi, A., Pandey, S. K., Sarma, N., & Lal, M. (2022). Induced variations by gamma radiation and EMS on the agronomic traits, essential oil yield with its quality and their exploitation in Java citronella (Cymbopogon winterianus Jowitt). International Journal of Radiation Biology, 98, 1376–1387.
Nash, R. J. (2000). Toxic plants: Dangerous to humans and animals. By Jean Bruneton. New Phytologist, 148, 57–58. https://doi.org/10.1046/j.1469-8137.2000.00735b.x
Negrelle, R. R. B., & Gomes, E. C. (2007). Cymbopogon citratus (DC.) Stapf: Chemical composition and biological activities. Revista Brasileira de Plantas Medicinais, 9, 80–92.
Nykanen, L., & Nykanen, I. (1987). The effect of drying on the composition of the essential oil of some Labiatae herbs cultivated in Finland. In Flavour science and technology. Wiley.
Okoh, O. O., Sadimenko, A. P., Asekun, O. T., & Afolayan, A. J. (2008). The effects of drying on the chemical components of essential oils of Calendula officinalis L. African Journal of Biotechnology, 7, 1500–1502.
Omidbaigi, R., Sefidkon, F., & Kazemi, F. (2004). Influence of drying methods on the essential oil content and composition of Roman chamomile. Flavour and Fragrance Journal, 19, 196–198.
Orphanides, A., Goulas, V., & Gekas, V. (2016). Drying technologies: Vehicle to high-quality herbs. Food Engineering Reviews, 8, 164–180. https://doi.org/10.1007/s12393-015-9128-9.
Ozdemir, N., Ozgen, Y., Kiralan, M., Bayrak, A., Arslan, N., & Ramadan, M. F. (2018). Effect of different drying methods on the essential oil yield, composition and antioxidant activity of Origanum vulgare L. and Origanum onites L. Journal of Food Measurement and Characterization, 12, 820–825. https://doi.org/10.1007/s11694-017-9696-x.
Padalia, R. C., Verma, R. S., Chanotiya, C. S., & Yadav, A. (2011). Chemical fingerprinting of the fragrance volatiles of nineteen Indian cultivars of Cymbopogon Spreng (Poaceae). Records of Natural Products, 5, 290.
Poehlmann, J. M., & Sleper, D. A. (1995). Breeding field crops. Iowa State University Press.
Rajashekar, Y., Kumar, H. V., Ravindra, K. V., & Bakthavatsalam, N. (2013). Isolation and characterization of biofumigant from leaves of Lantana camara for control of stored grain insect pests. Industrial Crops and Products, 51, 224–228.
Rajashekar, Y., Tonsing, N., Shantibala, T., & Manjunath, J. R. (2016). 2,3-Dimethylmaleic anhydride (3,4-dimethyl-2,5-furandione): A plant-derived insecticidal molecule from Colocasia esculenta var. esculenta (L.) Schott. Scientific Reports, 6, 20546.
Rocha, R. P., Melo, E. C., & Radünz, L. L. (2011). Influence of drying process on the quality of medicinal plants: A review. Journal of Medicinal Plant Research, 5, 7076–7084. https://doi.org/10.5897/JMPRx11.001.
Schaneberg, B. T., & Khan, I. A. (2002). Comparison of extraction methods for marker compounds in the essential oil of lemongrass by GC. Journal of Agricultural and Food Chemistry, 50, 1345–1349.
Shahi, A. K., Sharma, S. N., & Tava, A. (1997). Composition of Cymbopogon pendulus (Nees ex Steud.) Wats., an elemicin-rich oil grass grown in Jammu region of India. Journal of Essential Oil Research, 9, 561–563.
Shamsheer, H. B., Yousaf, Z., Aftab, A., Younas, A., Riaz, N., & Rashid, M. (2020). Phenotypic intraspecific diversity exploration among the accessions of Cymbopogon citratus (DC.) Stapf from Asia and American sub-continent through multivariate techniques. International Journal of Biology and Biotechnology, 17, 679–691.
Sidibé, L., Chalchat, J. C., Garry, R. P., Lacombe, L., & Harama, M. (2001). Aromatic plants of Mali (IV): Chemical composition of essential oils of Cymbopogon citratus (DC.) Stapf and C. giganteus (Hochst.) Chiov. Journal of Essential Oil Research, 13, 110–112.
Srivastava, R. K., Shukla, V. S., & Dagar, S. S. (2009). Cultivation and uses of aromatic plants. International Book Distributing Co. p. 138.
Venskutonis, P. R. (1997). Effect of drying on the volatile constituents of thyme (Thymus vulgaris L.) and sage (Salvia officinalis L.). Food Chemistry, 59, 219–227. https://doi.org/10.1016/S0308-8146(96)00242-7.
Verma, R. S., Verma, R. K., Yadav, A. K., & Chauhan, A. (2010). Changes in the essential oil composition of rose-scented geranium (Pelargonium graveolens L’Herit. ex Ait.) due to date of transplanting under hill conditions of Uttarakhand. Indian Journal of Natural Products and Resources, 1, 367–370.
Yogendra, N. D., Nazeer, M., Yadav, M. K., Baskaran, K., Pragadheesh, V. S., Jnanesha, A. C., & Srinivas, K. S. (2022). Comparative morphological assessment of lemongrass (Cymbopogon spp.) cultivars for oil yield, chemical composition and quality parameters under southern region of India. Madras Agricultural Journal, 108, 10–12.
Yousif, A. N., Scaman, C. H., Durance, T. D., & Girard, B. (1999). Flavor volatiles and physical properties of vacuum-microwave and air-dried sweet basil (Ocimum basilicum L.). Journal of Agricultural and Food Chemistry, 47, 4777–4781.
Zhang, L. L., Lv, S., Xu, J. G., & Zhang, L. F. (2018). Influence of drying methods on chemical compositions, antioxidant and antibacterial activity of essential oil from lemon peel. Natural Product Research, 32, 1184–1188.
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Devi, M. A., Singh, T. B., & Srivastava, R. K. (2023). Effect of drying techniques on essential oil yield and quality of lemongrass [Cymbopogon flexuosus (Nees ex Steud) Wats] cultivars Krishna and CKP-25. Journal of Medicinal and Aromatic Plant Sciences, 45(4), 143–151.
