Journal of Medicinal and Aromatic Plant Sciences

Volume: 45 Issue: 4

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  • Original Research Article

Phytochemical estimation and antimicrobial activity assessment of different solvent extracts of Tridax procumbens L.


Department of Biochemistry, Pt. JNM Medical College, Raipur, Chhattisgarh, India-492 001
*Corresponding author; E-mail: [email protected]

Year: 2023, Page: 123-132, Doi:

Received: Dec. 1, 2023 Accepted: Dec. 12, 2023 Published: Dec. 31, 2023


This study estimates the pharmacological properties of T. procumbens using qualitative and quantitative analysis of secondary metabolites, antioxidants, and antimicrobial activity analysis. Using standard methods, four aqueous extracts were prepared and screened for alkaloids, flavonoids, saponins, tannins, phenol, and ascorbic acid. The antioxidant profiling was undertaken with the support of 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical, ferric reducing antioxidant power (FRAP) and reducing power assay. The antimicrobial assay of crude fractions was conducted against human pathogens E. coli (MTCC 443) and Aspergillus niger (ITCC4718) using MIC analysis. Catalase (CAT), peroxidase (POX), superoxide dismutase (SOD) and ascorbate peroxidase (APX) activity was performed following standard methods for correlating antimicrobial properties with reactive oxygen species (ROS) scavenging activity. Tridax procumbens contains adequate amounts of carbohydrates and protein with some secondary metabolites. The extract also showed the presence of a significant quantity of antioxidants and satisfactory antimicrobial activity.

Keywords: Antimicrobial activity, Enzymatic antioxidants, Phytochemicals, Solvent extracts, Tridax procumbens


Ahmad P, Kumar A, Ashraf M, Akram, NA. 2012. Saltinduced changes in photosynthetic activity and oxidative defense system of three cultivars of mustard (Brassica juncea L.). Afr J Biotechnol 11: 2694-2703.

Ajali U, Okoye FBC. 2009. Antimicrobial and antiinflammatory activities of Olaxviridis root bark extracts and fractions. Int J Appl Res Nat Prod 2: 27-32.

Andriana Y, Xuan TD, Quy TN, Minh TN, Van TM, Viet TD. 2019. Anti-hyperuricemia, antioxidant, and antibacterial activities of Tridax procumbens L. Foods 8: 21.

Beara IN, Lesjak MM, Jovin ED, Balog KJ, Anackov GT, Orcic DZ, Mimica-Dukic NM. 2009. Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. J Agric Food Chem 57: 9268-9273.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.

Chandrasekaran M, Venkatesalu V. 2004. Antibacterial and antifungal activity of Syzygium jambolanum seeds. J Ethnopharmacol 91: 105-108.

Das K, Samant L, Chainy GBN. 2000. A modified spectrophotometric assay of superoxide dismutase using nitrite formation by superoxide radical. Ind J Biochem Biophy 37: 201-204.

Das MP, Dhanabalan R, Doss A, Palaniswamy M.2009. Phytochemical screening and antibacterial activity of aqueous and methanolic leaf extracts of two medicinal plants against bovine mastitis bacterial pathogens. Ethnobot leafl 2009: 131-139.

Gnanasekaran N, John JR, Sakthivel G, Kalavathy S. 2017. The comparative studies of the phytochemical levels and the in vitro antioxidant activity of Tridax procumbens L. from different habitats. Free Radic Antioxid 7: 50-56.

Ingole VV, Mhaske PC, Katade SR. 2022. Phytochemistry and pharmacological aspects of Tridax procumbens (L.): A systematic and comprehensive review. Phytomed Plus 2: 100199.

Jain A, Jain A. 2012 Tridax procumbens: A weed with immense medicinal importance: A review. Int J Pharma Bio Sci 3: 544-552.

Jain A, Rao VD, Jain A, Batra A. 2015. Enzymatic profiling and estimation of antioxidant potential in Tridax procumbens. Int J Adv Pharm Res 6: 137-146.

Katoch R. 2011. Analytical techniques in biochemistry and molecular biology, Springer-Verlag, New York, pp. 251.

Kuldeep G, Pathak AK. 2013. Pharmacognostic and phytochemical evaluation of Tridax procumbens Linn. J Pharmacogn Phytochem 1: 42-47.

Kumar S, Karl PN, Samuel J, Selvakumar M, Shalini K. 2016. Antioxidant, antidiabetic, antimicrobial and hemolytic activity of Tridax procumbens. J Chem Pharm Res 8: 808-812.

Kumari P, Joshi GC, Tewari LM. 2011. Diversity and status of ethno-medicinal plants of Almora district in Uttarakhand, India. Int J Biodivers Conserv 3: 298-326.

Lahlou M. 2013. The success of natural products in drug discovery. Pharmacol Pharma 4: 17-31.

Li P, Huo L, Su W, Lu R, Deng C, Liu L, Deng Y, Guo N, He, C. 2011. Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica. J Serb Chem Soc 76: 709-717.

Maksimović JJD, Živanović BD. 2012. Quantification of the antioxidant activity in salt-stressed tissues. In: Shabala S, Cuin TA, (Eds.). Plant Salt Tolerance: Methods and Protocols, Humana Press Publication, New Jersey, pp 237-350.

Mundada S, Shivhare R. 2010. Pharmacology of Tridax procumbens a weed. Int J Pharm Tech Res 2: 1391-1394.

Nair S, Dixit S, Ganesh N. 2016. A comparative analysis of in vitro antioxidant potential of crude extracts of Tridax procumbens L. in different solvents and in vitro hypoglycemic potential of its hydroalcoholic extract. Pharm Biomed Res 2: 47-55.

Naqash SY, Nazeer RA. 2011. Anticoagulant, antiherpetic, and antibacterial activities of sulphated polysaccharide from Indian medicinal plant Tridax procumbens L.(Asteraceae). Biotechnol Appl Biochem 165: 902-912.

Pai C, Kulkarni U, Borde M, Sowmya M, Mrudula P, Deshmukh Y. 2011. Antibacterial activity of Tridax procumbens with special reference to nosocomial pathogens. Br J Pharm Res 1: 164-173.

Patel NA, Vaidya SK, Kumar S, Prasad AK, Bothara SB.2014. Antioxidant and hepatoprotective activity of extracts of flowers of Tridax procumbens L., against D-galectosamine induced hepatotoxicity in male wister albino rats. Indo Am J pharm Sci 4: 3712-3720.

Pauline N, Cabral BNP, Anatole PC, Jocelyne AMV, Bruno M, Jeanne NY. 2013. The in vitro antisickling and antioxidant effects of aqueous extracts Zanthoxyllum heitzii on sickle cell disorder. BMC Complement Altern Med 13: 162.

Rani P, Unni K, Karthikeyan J. 2004. Evaluation of antioxidant properties of berries. Indian J Clin Biochem 19: 103-110.

Rao JK. 2015. Antifungal potential of some medicinal plants against seed-borne fungi. Int Res J Sci Eng 3: 28-32.

Samal J. 2016. Medicinal plants and related developments in India: A peep into 5-year plan of India. Ind J Health Sci 9: 14-19.

Sawant RS, Godghate AG. 2013. Preliminary phytochemical analysis of leaves of Tridax procumbens Linn. In J Sci Environ Technol 2: 388-394.

Taddei A, Rosas-Romero AJ. 2000. Bioactivity studies of extracts from Tridax procumbens. Phytomed 7: 235-238.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. 2011. Phytochemical screening and extraction: a review. Int Pharm Sci 1: 98-106.

Uzuegbu U, Mordi J, Ovuakporaye S, Ewhre LOE.2016. Effects of aqueous and ethanolic extracts of Tridax procumbens leaves on gastrointestinal motility and castor oil-induced diarrhoea in wistar rats. Biokemistri 27: 26-32.

Yadav RNS, Agarwala M. 2011. Phytochemical analysis of some medicinal plants. J Phytol 3: 10-14.

Cite this article

Joshi A, Patra PK, Mishra PK, Khodiar PK, Sahu GK.2023. Phytochemical estimation and antimicrobial activity assessment of different solvent extracts of Tridax procumbens L. J Med Aromat Plant Sci 45: 123-132.