Volume: 37 Issue: 3
The content, profile and biological activity of tannins in some tanniniferous plants
Year: 2015, Page: 46-51, Doi: https://doi.org/10.62029/jmaps.v37i3.Bharti
Received: Aug. 28, 2014 Accepted: Feb. 10, 2015 Published: Dec. 31, 2015
The present study was carried out on the quantification, chromatographic profiling and biological activity assessments of selected tanniniferous forages viz. leaves of oak/ban (Quercus leucotrichophora), robinia (Robinia pseudoacacia), khirk (Celtisaustralis), kachnar (Bauhinia variegata), siris (Albizia lebbeck), pakar (Ficus infectoria), tremal (Ficus roxburghii) and buince (Salix alba), and leaves and fruits of bhera (Terminalia bellerica) and harad (Terminalia chebula). The total tannin content was found high in harad and bhera fruits & leaves. Kachnar, robinia and pakar leaves were rich source of condensed tannins. However, hydrolysable tannins were the predominant component of the total tannin phenols of oak, harad and bhera leaves. The extraction of tannins was followed by TLC fingerprinting. TLC fingerprint analysis of selected plants showed remarkable difference in the overall complexity and the polarity of the tannins. Prospections were also carried out on the protein precipitating capacity and free radical scavenging activity of these tannins. The antioxidant activity of the plant samples was done using 1,1-Diphenyl-2-picryl-hydrazyl spray. The protein precipitation capacity and antioxidant activity – the major indicators of biological activity of tannins, suggested that a fine balance has to be struck between the beneficial and deleterious effects of these tannins by maintaining a control over their concentration vis-à-vis their intake by the animals.
Keywords: Biological activity, Chromatographic profile, Tannins
Asquith RA, Butler LG. 1985. Use of dye labeled protein as spectrophotometric assay for protein precipitant as tannins. J Chem Ecol 11: 1535-1544.
Barry TN, McNabb WC. 1999. The implications of condensed tannins on the nutritive value of temprate forages fed to ruminants. Brit J Nutr 81: 263-272.
Dhar J, Sharma OP, Bhat TK, Kannan A.2012. An improved protocol for isolation of tannins, and their thin layer chromatographic fingerprint analysis. Animal Nutrition Research Strategies for Food Security. In Proc. 8th Biennial Anim Nutr Assoc Conf, 28-30 November, 2012, Bikaner, Rajasthan. Abstr. No. 28, p 67.
Hagerman AE, Butler LG. 1981. The specificity of proanthocyanidin – protein interactions. J Biol Chem 256: 4494-4497.
Kordali S, Cakir A, Mavi A, Killic H, Yildrim A.2005. Screening of chemical composition and antifungal and antioxidant activities of essential oils from three Turkish Artemisia species. J Agric Food Chem 53: 1408-1416.
Makkar HPS. 2003. Quantification of tannins in tree and shrub foliage - A laboratory manual. Kluwer Academic Publishers, Sordrecht, Netherlands, pp 43-54.
Mueller-Harvey I, Mlambo V, Sikosana JL, Smith T, Owen E, Brown RH. 2007. Octanolwater partition coefficients for predicting the effects of tannins in ruminant nutrition. J Agric Food Chem 55: 5436-5444.
Muller-Harvey I. 2006. Unravelling the conundrum of tannins in animal nutrition and health. J Sci Food Agric 86: 2010-2037.
Patra AK, Saxena J. 2011. Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition. J Sci Food Agric 91: 24-37.
Reed JD, Horvath PJ, Allen MS, Van Soest PJ. 1985. Gravimetric determination of soluble phenolics including tannins from leaves by precipitation with trivalent ytterbium. J Sci Food Agric 36: 255-261.
Sharma OP, Bhat TK. 2009. DPPH antioxidant assay revisited. Food Chem 113: 1202-1205.
Stahl E. 1969. Thin Layer Chromatography -A Laboratory Handbook. Springer International Student Edition, pp. 167-181.
© Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow (India)
Anu Bharti, Jyoti Babu Dhar, Tej Krishan Bhat. 2015. The content, profile and biological activity of tannins in some tanniniferous plants. J Med Aromat Plant Sci 37: 46-51.