Volume: 43 Issue: 1
Determination of multi-bioactive compounds in the root of Ricinus communis using HPLC- Evaporative Light Scattering Detection (HPLC-ELSD) method for quality control.
Year: 2021, Page: 1-8, Doi: https://doi.org/10.62029/jmaps.v43i1.Shanker
Received: March 17, 2021 Accepted: April 28, 2021 Published: June 30, 2021
First time, a simple, and reliable method based on HPLC coupled to an evaporative light scattering detector (ELSD) was established for the simultaneous determination of triterpenes and phytosterols viz. erandone (urs-6-ene-3,16-dione) (1), lupeol (2), stigmasterol (3) and sitosterol (4) in Ricinus communis (Erand) root, an important traditional medicinal plant used in Indian System of medicine. The chromatographic separation was performed on a Phenonemex Luna-C18 (4.6mm × 50mm, 3?m) using isocratic elution of 1% acetic acid in acetonitrile and 1% acetic acid in water. Diverse method validation parameters, such as the linearity (r > 0.999), sensitivities (LOD and LOQ), accuracy, precision, repeatability, and reproducibility, were successfully obtained. These four phytochemicals (1-4) including one new diketone pentacyclic triterpene (erandone) show potential as marker compounds for evaluating the quality of R. communis and the variation in its chemical composition due to geographical-environmental interaction.
Keywords: HPLC-ELSD, Erandone (urs-6-ene-3, 16-dione), lupeol, Ricinus communis
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Pooja Srivastava, Jyotsna, Princi Gupta, Shraddha Rahate, Pooja Gaur, Ranjana, Ramdas, Karuna Shanker. 2021. Determination of multi-bioactive compounds in the root of Ricinus communis using HPLC- Evaporative Light Scattering Detection (HPLC-ELSD) method for quality control. J Med Aromat Plant Sci 43 : 1-8.
