Volume: 43 Issue: 3
Dactyloidin from Myristica fatua Houtt. seed enhances glucose uptake through translocation of GLUT4 via upregulation of AMPK in L6 myotubes
Year: 2021, Page: 108-116, Doi: https://doi.org/10.62029/jmaps.v43i3.Prabha
Received: June 12, 2021 Accepted: Oct. 12, 2021 Published: Dec. 31, 2021
Dactyloidin (DTN), a diarylnonanoid, was isolated for the first time from the seeds of Myristica fatua Houtt. DTN displayed significant inhibitory activity on α-glucosidase enzyme with IC50 value of 55.08 ± 0.857 µM. Molecular docking studies showed that DTN effectively binds to the active sites of N- terminal human maltase glucoamylase (3TOP), which supported the observed α-glucosidase inhibition. DTN also exhibited an enhanced glucose uptake in L6 myotubes with 31.5%. Exhilarated with these observations, we investigated the molecular mechanism of DTN by investigating the modulation of AMPK. The results revealed that DTN increased the glucose uptake in L6 myotubes by stimulating the translocation and expression of GLUT4.
Keywords: AMPK pathway, Dactyloidin, Glucose uptake, GLUT4 translocation, Human maltase glucoamylase, -glucosidase inhibition
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© CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015
Prabha B, Sini S, Sherin DR, Neethu S, Govind MG, Dan M, Manojkumar TK, Jayamurthy P, Radhakrishnan KV. 2021. Dactyloidin from Myristica fatua Houtt. seed enhances glucose uptake through translocation of GLUT4 via upregulation of AMPK in L6 myotubes. J Med aromat Plant Sci 43: 108-116.
