Volume: 43 Issue: 3
Bacillus cereus ApKb1 associated with the damping-off disease on Andrographis paniculata in India
Year: 2021, Page: 127-132, Doi: https://doi.org/10.62029/jmaps.v43i3.Bandamaravuri
Received: Oct. 5, 2021 Accepted: Nov. 11, 2021 Published: Dec. 31, 2021
During the early rainy season (June-July) of 2018 and 2019, seedlings in A. paniculata nursery at CIMAP experimental field were found dying due to damping-off disease. The symptoms comprised basal stem lesions spreading upward, making the stem become wired at a later stage and falling off due to premature death. The damping-off disease incidence on A. paniculata nursery was estimated as 25% and 30% during 2018 and 2019, respectively. A few bacteria were isolated from the diseased plantlets, and pathogenicity assays were performed by spray inoculation of bacterial suspension (2 ×104 CFU/ml) on 7-10 day old plantlets of A. paniculata. Our results indicated a strong association of ApKb1 isolate to damping-off symptoms on A. paniculata, and the microscopic observations revealed that the pathogen was Gram-positive, rod-shaped, and 0.30-0.38 µm in size. Further identification of the pathogen, based on 16S rDNA sequencing and BLASTn homology search of partial 16S rDNA sequence (1374bp), showed 99% similarity with Bacillus cereus strain DD123. This is the first report of the damping-off disease of A. paniculata associated with Bacillus cereus ApKb1 in India, to the best of our knowledge.
Keywords: Andrographis paniculata, Bacillus cereus, Damping-off, Soil-borne bacteria
Akbar, S. (2011). Andrographis paniculata: A review of pharmacological activities and clinical effects. Alternative Medicine Review, 16, 66–77.
Barbetti, M. J., Riley, I. T., You, M. P., Li, H., & Sivasithamparam, K. (2007). Management of root diseases of annual pasture legumes in Mediterranean ecosystems—a case study of subterranean clover root diseases in the southwest of Western Australia. Phytopathology Mediterranean, 46, 239–258.
Bottone, E. J. (2010). Bacillus cereus, a volatile human pathogen. Clinical Microbiology Reviews, 23, 382–398.
Burkill, I. H., Birtwistle, W., Foxworthy, F., Scrivenor, J., & Watson, J. (1966). A dictionary of the economic products of the Malay Peninsula. Ministry of Agriculture and Co-operatives.
Foster, K., You, M. P., Nietschke, B., Edwards, N., & Barbetti, M. J. (2017). The widespread decline of subterranean clover pastures across diverse climatic zones is driven by soil-borne root disease pathogen complexes. Crop and Pasture Science, 68, 33–44.
Fredriksson, N. J., Hermansson, M., & Wilén, B. M. (2013). The choice of PCR primers has a great impact on assessments of bacterial community diversity and dynamics in a wastewater treatment plant. PLOS ONE, 8, e76431.
Gupta, S., Choudhry, M. A., Yadava, J. N. S., Srivastava, V., & Tandon, J. S. (1990). Antidiarrhoeal activity of diterpenes of Andrographis paniculata (Kalmegh) against Escherichia coli enterotoxin in vivo models. International Journal of Crude Drug Research, 28, 273–283.
Jarukamjorn, K., & Nemoto, N. (2008). Pharmacological aspects of Andrographis paniculata on health and its major diterpenoid constituent andrographolide. Journal of Health Science, 54, 370–381.
Kumar, R. A., Sridevi, K., Kumar, N. V., & Nanduri, S. R. (2004). Anti-cancer and immunostimulatory compounds from Andrographis paniculata. Journal of Ethnopharmacology, 92, 291–295.
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.
Kumar, V., Thakur, A. K., & Chatterjee, S. S. (2014). The perspective of Andrographis paniculata in neurological disorders. Clinical Pharmacology & Biopharmaceutics, 2, 2.
Lamichhane, J. R., Durr, C., Schwanck, A. A., Robin, M. H., Sarthou, J. P., Cellier, V., Mesean, A., & Aubertot, J. N. (2017). Integrated management of damping-off diseases: A review. Agronomy for Sustainable Development, 37, 10.
Lamichhane, J. R., & Venturi, V. (2015). Synergisms between microbial pathogens in plant disease complexes: A growing trend. Frontiers in Plant Science, 6, 385.
Liu, B., Shen, W., Wei, H., Smith, H., Louws, F. J., Steadman, J. R., & Correll, J. C. (2016). Rhizoctonia communities in soybean fields and their relation with other microbes and nematode communities. European Journal of Plant Pathology, 144, 671–686.
Matoba, Y., Kondo, N., Akino, S., Kodama, F., Naito, S., & Ebe, S. (2008). Identification and pathogenicity of Pythium species causing damping-off of kidney bean. Journal of General Plant Pathology, 74, 81.
McDowell, R. H., Sands, E. M., & Friedman, H. (2019). Bacillus cereus. In StatPearls [Internet]. StatPearls Publishing. PMID: 29083665.
Mishra, S. K., Sangwan, N. S., & Sangwan, R. S. (2007). Andrographis paniculata (Kalmegh): A review. Pharmacognosy Reviews, 1, 283–298.
Misra, P., Pal, N. L., Guru, P. Y., Katiyar, J. C., Srivastava, V., & Tandon, J. S. (1992). Antimalarial activity of Andrographis paniculata (Kalmegh) against Plasmodium berghei NK65 in Mastomys natalensis. International Journal of Pharmaceutical and Phytochemical Research, 13, 263–274.
Nanduri, S., Nyavanandi, V. K., Thunuguntla, S. S., Kasu, S., Pallerla, M. K., Ram, P. S., Rajagopal, S., Kumar, R. A., Ramanujam, R., Babu, J. M., Vyas, K., Devi, A. S., Reddy, G. O., & Akella, V. (2004). Synthesis and structure–activity relationships of andrographolide analogs as novel cytotoxic agents. Bioorganic & Medicinal Chemistry Letters, 14, 4711–4717.
Navi, S. S., Huynh, T., Mayers, C. G., & Yang, X. B. (2019). Diversity of Pythium spp. associated with soybean damping-off and management implications by using foliar fungicides as seed treatments. Phytopathology Research, 1, 1–10.
Niranjan, A., Tewari, S. K., & Lehri, A. (2010). Biological activities of kalmegh (Andrographis paniculata Nees). Indian Journal of Natural Products and Resources, 1, 125–135.
Okhuarobo, A., Falodun, J. E., Erharuyi, O., Imieje, V., Falodun, A., & Langer, P. (2014). Harnessing the medicinal properties of Andrographis paniculata for diseases and beyond: A review of its phytochemistry and pharmacology. Asian Pacific Journal of Tropical Disease, 4, 213.
Rojas, J. A., Jacobs, J. L., Napieralski, S., Karaj, B., Bradley, C. A., Chase, T., Esker, P. D., Giesler, L. J., Jardine, D. J., Malvick, D. K., & Markell, S. G. (2016). Oomycete species associated with soybean seedlings in North America—Part II: Diversity and ecology in relation to environmental and edaphic factors. Phytopathology, 107, 293–304.
Sharma, A., Singh, R. T., Sehgal, V., & Handa, S. S. (1991). The antihepatotoxic activity of some plants used in herbal formulations. Fitoterapia, 62, 131–138.
Tan, B. K., & Zhang, A. C. (2004). Andrographis paniculata and the cardiovascular system. In Herbal Traditional Medicine (Vol. 1, pp. 399–415). CRC Press.
Verma, H., Negi, M. S., Mahapatra, B. S., & Paul, A. S. (2019). Evaluation of an emerging medicinal crop kalmegh [Andrographis paniculata (Burm. f.) Wall. ex Nees] for commercial cultivation and pharmaceutical and industrial uses: A review. Journal of Pharmacognosy and Phytochemistry, 8, 835–848.
You, M. P., Rensing, K., Renton, M., & Barbetti, M. J. (2017). Modeling effects of temperature, soil moisture, nutrition, and variety as determinants of severity of Pythium damping-off and root disease in subterranean clover. Frontiers in Microbiology, 8, 22–23.
© CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015
Bandamaravuri, K. B., Sharma, B., Bandamaravuri, A. S., Tiwari, N., & Samad, A. (2021). Bacillus cereus ApKb1 associated with the damping-off disease on Andrographis paniculata in India. Journal of Medicinal and Aromatic Plant Sciences, 43(3), 127–132.
