Journal of Medicinal and Aromatic Plant Sciences

Volume: 46 Issue: 1

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  • Original Research Article

Comparative studies on variation of β-Asarone composition in Acorus calamus Linn. by GC and HPLC

Munigela Sumalatha1#, Arigari Niranjana Kumar2#, Jonnala Kotesh Kumar2*, Kalavagunta Venkata Naga Satya Srinivas2, Miriyala Vijay Kumar2, Kommineni Vinutha1

1Sri Venkateswara College of Pharmacy, Hyderabad, India.
2Phytochemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Boduppal, Hyderabad, India.
#M. Sumalatha and A. Niranjana Kumar have equal contributions and considered as first authors.

*Corresponding author; email: [email protected]

Year: 2024, Page: 17-22, Doi: https://doi.org/10.62029/jmaps.v46i1.sumalatha

Received: March 12, 2024 Accepted: May 10, 2024 Published: May 10, 2024

Abstract

The objective of this study was to assess the essential oil yields and chemical composition of the root of two varieties of Acorus calamus, namely the local and Jabalpur varieties. Gas chromatography (GC) and high-performance liquid chromatography (HPLC) analysis identified β-Asarone as the major chemical constituent. Notable findings revealed that fresh samples exhibited higher essential oil yield and β-Asarone content compared to various drying methods. Specifically, powdering the Jabalpur material resulted in a significant percentage (2.0%) of essential oils, with negligible variations observed in β-Asarone content. Additionally, besides GC, a straightforward and effective reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed for β-Asarone analysis from A. calamus root extracts in different solvents. Interestingly, the spent material also contained a considerable amount of β-Asarone. Among the different solvent extractions, ethyl acetate extracts exhibited the highest percentage (62%) of β-Asarone, while the lowest amount was recorded in methanol extract (37.47%).

Keywords: Acorus calamus, Essential oil, GC, HPLC, β-Asarone.

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Cite this article

Munigela, S., Arigari, N. K., Jonnala, K. K., Kalavagunta, V. N. S., Miriyala, V. K., & Kommineni, V. (2024). Comparative studies on variation of β-Asarone composition in Acorus calamus Linn. by GC and HPLC. Journal of Medicinal and Aromatic Plants, 46(1), 17-22. https://doi.org/10.62029/jmaps.v46i1.sumalatha

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