Volume: 43 Issue: 1
Bioassay-guided isolation and characterization of potential α-glucosidase inhibitors from the seeds of Quassia indica Gaetrn.
Year: 2021, Page: 25-36, Doi: https://doi.org/10.62029/jmaps.v43i1.B
Received: June 12, 2021 Accepted: June 20, 2021 Published: July 1, 2021
Bioactivity-guided isolation afforded 10 compounds from the ethanolic extract of the seeds of Quassia indica including, four novel quassinoids namely; samaderin L (6), samaderin R (7), samaderoside L (9) and samaderoside R (10). The structures were illustrated based on 1D and 2D NMR data, HRESIMS and singlecrystal X-ray analysis. Among the quassinoids, samaderoside R (10) displayed significant α-glucosidase inhibitory activity. Molecular simulation experiments proved that samaderoside R (10) effectively binds to the active sites of Saccharomyces cerevisiae (3A4A) and human maltase glucoamylase (N- and C-terminal: 2QMJ and 3TOP) enzymes.
Keywords: Molecular simulation studies, Quassia indica Gaetrn, Quassinoids, -Glucosidase inhibitory activity
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