Volume: 43 Issue: 3
Bacillus cereus ApKb1 associated with the damping-off disease on Andrographis paniculata in India
Year: 2021, Page: 127-132, Doi: https://doi.org/10.62029/jmaps.v43i3.Bandamaravuri
Received: Oct. 5, 2021 Accepted: Nov. 11, 2021 Published: Dec. 31, 2021
During the early rainy season (June-July) of 2018 and 2019, seedlings in A. paniculata nursery at CIMAP experimental field were found dying due to damping-off disease. The symptoms comprised basal stem lesions spreading upward, making the stem become wired at a later stage and falling off due to premature death. The damping-off disease incidence on A. paniculata nursery was estimated as 25% and 30% during 2018 and 2019, respectively. A few bacteria were isolated from the diseased plantlets, and pathogenicity assays were performed by spray inoculation of bacterial suspension (2 ×104 CFU/ml) on 7-10 day old plantlets of A. paniculata. Our results indicated a strong association of ApKb1 isolate to damping-off symptoms on A. paniculata, and the microscopic observations revealed that the pathogen was Gram-positive, rod-shaped, and 0.30-0.38 µm in size. Further identification of the pathogen, based on 16S rDNA sequencing and BLASTn homology search of partial 16S rDNA sequence (1374bp), showed 99% similarity with Bacillus cereus strain DD123. This is the first report of the damping-off disease of A. paniculata associated with Bacillus cereus ApKb1 in India, to the best of our knowledge.
Keywords: Andrographis paniculata, Bacillus cereus, Damping-off, Soil-borne bacteria
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© CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015
Bandamaravuri KB, Sharma B, Bandamaravuri AS, Tiwari N, Samad A. 2021. Bacillus cereus ApKb1 associated with the damping-off disease on Andrographis paniculata in India. J Med Aromat Plant Sci 43: 127-132.
