Volume: 46 Issue: 1
New and emerging trends in phytopathology of medicinally bioactive geographical indicator of Kashmir: Saffron (Crocus sativus L.)
Year: 2024, Page: 10-16, Doi: https://doi.org/10.62029/jmaps.v46i1.shahnaz
Received: Dec. 20, 2023 Accepted: Feb. 19, 2024 Published: Feb. 19, 2024
Saffron (Crocus sativus L.) is an important geographical indicator (GI) tagged cash crop from the Kashmir valley of India, making it a global brand. This spice crop, valued for its dried stigma with a characteristic odor and coloration, is most sought after for its diverse pharmacological activities (anti-tumor, anti-cancer, anti-aging, and their use in cosmeceuticals and pharmaceuticals). Saffron is used against diseases of the central nervous system, age-related diseases, ocular diseases and neuro-degenerative diseases. India contributes 5% of global saffron production and 90% of this produce comes from Jammu and Kashmir. However, the yield of 2.0 to 2.5 kg/ha is very low compared to the global average of 3.4 kg/ha. This has been attributed mainly to the loss of genetic yield potential due to vegetative propagation, primitive cultivation practices, dependence on natural climatic conditions, and stress caused by abiotic and biotic factors. Among the biotic factors, corm rot is the most severe disease, causing a drastic reduction in yields due to the compounding effect of the causal pathogens over the years. The current review has highlighted some of the biological control measures that have been adopted in recent years for the management of this disease. Many new diseases reported in the last 20 years have also been highlighted. The paper presents some of the futuristic trends in saffron research.
Keywords: Saffron, Phytopathology, Biocontrol, Futuristic trends, Bioactivity.
Abdullaev, F. I., & Espinosa-Aguirre, J. J. (2004). Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detection and prevention, 28(6), 426-432.
Ahmad, M., Sagar, V., Shah, M. U. D., Padder, B. A., Ahanger, F. A., Sofi, T. A., ... & Khan, M. A. (2012, October). Management of corm rot of saffron (Crocus sativus L.) in Kashmir, India. In IV International Symposium on Saffron Biology and Technology (Vol. 1200, pp. 111–114).
Ahmad, T., Bashir, A., Farooq, S., & Riyaz‐Ul‐Hassan, S. (2022). Burkholderia gladioli E39CS3, an endophyte of Crocus sativus Linn., induces host resistance against corm‐rot caused by Fusarium oxysporum. Journal of Applied Microbiology, 132(1), 495–508.
Baba, S. A., Malik, A. H., Wani, Z. A., Mohiuddin, T., Shah, Z., Abbas, N., & Ashraf, N. (2015). Phytochemical analysis and antioxidant activity of different tissue types of Crocus sativus and oxidative stress alleviating potential of saffron extract in plants, bacteria, and yeast. South African Journal of Botany, 99, 80–87.
Bagherzade, G., Tavakoli, M. M., & Namaei, M. H. (2017). Green synthesis of silver nanoparticles using aqueous extract of saffron (Crocus sativus L.) wastages and its antibacterial activity against six bacteria. Asian Pacific Journal of Tropical Biomedicine, 7(3), 227–233.
Bhagat, N., & Vakhlu, J. (2024). Effects of biocontrol Bacillus sp. strain D5 on the pathogenic Fusarium oxysporum R1 at the microscopic and molecular level in Crocus sativus L.(saffron) corm. FEMS microbes, 5, xtad025.
Caballero-Ortega, H., Pereda-Miranda, R., & Abdullaev, F. I. (2007). HPLC quantification of major active components from 11 different saffron (Crocus sativus L.) sources. Food Chemistry, 100(3), 1126–1131.
Cardone, L., Castronuovo, D., Perniola, M., Cicco, N., & Candido, V. (2020). Saffron (Crocus sativus L.), the king of spices: An overview. Scientia Horticulturae, 272, 109560.
Christodoulou, E., Kadoglou, N. P., Kostomitsopoulos, N., & Valsami, G. (2015). Saffron: a natural product with potential pharmaceutical applications. Journal of Pharmacy and Pharmacology, 67(12), 1634–1649.
Del Gallo, M., Farda, B., Djebaili, R., Sabbi, E., Ercole, C., Pace, L., & Pellegrini, M. (2023, May). Microbial communities investigation and biocontrol activity in saffron cultures. In EGU General Assembly Conference Abstracts (pp. EGU–16433).
Dhar, A. K. (2000). Saffron: biology, utilization, agriculture, production and quality. Science Publishers, Inc.
Du, S., Lu, J., Hu, B., Han, T., Kong, Z., Qin, L., & Zhu, B. (2023). Endophytic fungi prevent corm rot disease and stimulate the growth and metabolism of Crocus sativus. Applied Biochemistry and Microbiology, 59(4), 503–510.
Fiori, M., Falchi, G., Quaglia, M., & Cappelli, C. (2007). Saffron (Crocus sativus L.) diseases in Italy. Journal of Plant Pathology, 89, 41.
Fiori, M., Ligios, V., & Schiaffino, A. (2011). Identification and characterization of Burkholderia isolates obtained from bacterial rot of saffron (Crocus sativus L.) grown in Italy. Phytopathologia Mediterranea, 50(3), 450–461.
Gupta, R., & Vakhlu, J. (2015). Native Bacillus amyloliquefaciens W2 as a potential biocontrol for Fusarium oxysporum R1 causing corm rot of Crocus sativus. European Journal of Plant Pathology, 143, 123–131.
Gupta, V., Kumar, K., Fatima, K., Razdan, V. K., Sharma, B. C., Mahajan, V., ... & Hussain, R. (2020). Role of biocontrol agents in management of corm rot of saffron caused by Fusarium oxysporum. Agronomy, 10(9), 1398.
Gupta, V., Sharma, A., Rai, P. K., Gupta, S. K., Singh, B., Sharma, S. K., ... & Sharma, R. (2021). Corm rot of saffron: Epidemiology and management. Agronomy, 11(2), 339.
Hausenblas, H. A., Saha, D., Dubyak, P. J., & Anton, S. D. (2013). Saffron (Crocus sativus L.) and major depressive disorder: a meta-analysis of randomized clinical trials. Journal of Integrative Medicine, 11(6), 377–383.
Hossainnia, A., & Mohammadi, A. (2018). Investigation of Alternaria alternata pathogenicity on corm and leaves of saffron in-vitro and greenhouse conditions. Saffron Agronomy and Technology, 6(1), 61–72. https://doi.org/10.22048/jsat.2016.54452.1164
Hu, S., Sun, W., Wang, X., Wang, L., & Li, W. (2022). First report of black spot caused by Penicillium citreosulfuratum on saffron in Chongming Island, China. Plant Disease, 106(2), 760.
Hu, S., Wang, X., Sun, W., Wang, L., & Li, W. (2021). In vitro study of biocontrol potential of rhizospheric Pseudomonas aeruginosa against pathogenic fungi of saffron (Crocus sativus L.). Pathogens, 10(11), 1423.
Joukar, S., Najafipour, H., Khaksari, M., Sepehri, G., Shahrokhi, N., Dabiri, S., ... & Hasanzadeh, S. (2010). The effect of saffron consumption on biochemical and histopathological heart indices of rats with myocardial infarction. Cardiovascular Toxicology, 10, 66–71.
Kalha, C. S., Gupta, V., Gupta, D., & Priya, S. (2007). First report of sclerotial rot of saffron caused by Sclerotium rolfsii in India. Plant Disease, 91(9), 1203-1203.
Khezri, M., Shahri, M. R. K., & Ghasem, A. (2023). Bacterial rot disease of saffron corm and leaf. Plant Pathology Science, 12(1), 74–83.
Khoulati, A., Ouahhoud, S., Mamri, S., Alaoui, K., Lahmass, I., Choukri, M., ... & Saalaoui, E. (2019). Saffron extract stimulates growth, improves the antioxidant components of Solanum lycopersicum L., and has an antifungal effect. Annals of Agricultural Sciences, 64(2), 138–150.
Kumar, A., Devi, M., Kumar, R., & Kumar, S. (2022). Introduction of high-value Crocus sativus (saffron) cultivation in non-traditional regions of India through ecological modelling. Scientific Reports, 12(1), 11925.
Leone, S., Recinella, L., Chiavaroli, A., Orlando, G., Ferrante, C., Leporini, L., ... & Menghini, L. (2018). Phytotherapic use of the Crocus sativus L.(Saffron) and its potential applications: A brief overview. Phytotherapy Research, 32(12), 2364–2375.
Makhlouf, H., Saksouk, M., Habib, J., & Chahine, R. (2011). Determination of antioxidant activity of saffron taken from the flower of Crocus sativus grown in Lebanon. African Journal of Biotechnology, 10(41), 8093–8100.
Mansotra, R., Ali, T., Bhagat, N., & Vakhlu, J. (2023). Injury and not the pathogen is the primary cause of corm rot in Crocus sativus (saffron). Frontiers in Plant Science, 14, 1074185.
Mirghasempour, S. A., Studholme, D. J., Chen, W., Cui, D., & Mao, B. (2022). Identification and characterization of Fusarium nirenbergiae associated with saffron corm rot disease. Plant Disease, 106(2), 486–495.
Moghaddasi, M. S. (2010). Saffron chemicals and medicine usage. Journal of Medicinal Plants Research, 4(6), 427–430.
Mottaghipisheh, J., Sourestani, M. M., Kiss, T., Horváth, A., Tóth, B., Ayanmanesh, M., ... & Csupor, D. (2020). Comprehensive chemotaxonomic analysis of saffron crocus tepal and stamen samples, as raw materials with potential antidepressant activity. Journal of Pharmaceutical and Biomedical Analysis, 184, 113183.
Mousavi, S. Z., & Bathaie, S. Z. (2011). Historical uses of saffron: Identifying potential new avenues for modern research. Avicenna Journal of Phytomedicine, 1(2), 57–66.
Muñoz, R. M., Lerma, M. L., Castillo, P., Armengol, J., Somoza, E., & Woodhall, J. W. (2020). First report of Stromatinia gladioli causing neck and corm rot of Crocus sativus in Spain. Plant Disease, 104(1), 282.
Mzabri, I., Addi, M., & Berrichi, A. (2019). Traditional and modern uses of saffron (Crocus sativus). Cosmetics, 6(4), 63.
Najari, G., & Nourollahi, K. (2019). Genetic diversity of Alternaria alternata the causal agent of saffron corm rot in southern and Razavi Khorasan provinces using microsatellite markers. Saffron Agronomy and Technology, 7(3), 331–346
Ochiai, T., Shimeno, H., Mishima, K. I., Iwasaki, K., Fujiwara, M., Tanaka, H., ... & Soeda, S. (2007). Protective effects of carotenoids from saffron on neuronal injury in-vitro and in-vivo. Biochimica et Biophysica Acta (BBA)-General Subjects, 1770(4), 578–584.
Papandreou, M. A., Tsachaki, M., Efthimiopoulos, S., Cordopatis, P., Lamari, F. N., & Margarity, M. (2011). Memory enhancing effects of saffron in aged mice are correlated with antioxidant protection. Behavioural Brain Research, 219(2), 197–204.
Premkumar, K., Thirunavukkarasu, C., Abraham, S. K., Santhiya, S. T., & Ramesh, A. (2006). Protective effect of saffron (Crocus sativus L.) aqueous extract against genetic damage induced by anti-tumor agents in mice. Human and Rxperimental Toxicology, 25(2), 79–84.
Rasool, A., Mir, M. I., Zulfajri, M., Hanafiah, M. M., Unnisa, S. A., & Mahboob, M. (2021). Plant growth promoting and antifungal asset of indigenous rhizobacteria secluded from saffron (Crocus sativus L.) rhizosphere. Microbial Pathogenesis, 150, 104734.
Rubio-Moraga, Á., Gómez-Gómez, L., Trapero, A., Castro-Díaz, N., & Ahrazem, O. (2013). Saffron corm as a natural source of fungicides: The role of saponins in the underground. Industrial Crops and Products, 49, 915–921.
Sameer, S. S., Bashir, S., Nehvi, F. A., Iqbal, A. M., Naseer, S., Nagoo, S. A., & Dar, N. A. (2012, October). Effect of biofertilizers, biological control agents and soil amendments on the control of saffron corm rot (Crocus sativus L.). In IV International Symposium on Saffron Biology and Technology (Vol. 1200, pp. 121–124).
Shah, M. U. D., Ahmad, M., Sagar, V., Padder, B. A., Ahanger, F. A., Sofi, T. A., ... & Kausar, S. (2012, October). In-vitro evaluation of bioagents and fungitoxicants against Fusarium oxysporum and Fusarium solani causing corm rot of saffron (Crocus sativus) in Kashmir, India. In IV International Symposium on Saffron Biology and Technology (Vol. 1200, pp. 125–132).
Shahnaz, E., Misger, F. A., & Kumar, A. (2013). On farm treatment on corm rot of saffron (Crocus sativus L.) in Pulwama district of Kashmir valley. Research Journal of Agricultural Sciences, 4(1).
Shuwen, H., Qing, Z., Jin, L., & Miao, D. (2021). Fungal diversity on the surface of saffron corms with different growth characteristics. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 155(2), 302–309.
Tian, L., Hu, S., Wang, X., Guo, Y., Huang, L., Wang, L., & Li, W. (2022). Antagonism of rhizosphere Streptomyces yangpuensis CM253 against the pathogenic fungi causing corm rot in saffron (Crocus sativus L.). Pathogens, 11(10), 1195.
Tuberoso, C. I., Rosa, A., Montoro, P., Fenu, M. A., & Pizza, C. (2016). Antioxidant activity, cytotoxic activity and metabolic profiling of juices obtained from saffron (Crocus sativus L.) floral by-products. Food Chemistry, 199, 18–27.
Zhang, A., Shen, Y., Cen, M., Hong, X., Shao, Q., Chen, Y., & Zheng, B. (2019). Polysaccharide and crocin contents, and antioxidant activity of saffron from different origins. Industrial Crops and Products, 133, 111–117.
Zhang, T., Huang, C., Deng, C., Zhang, Y., Feng, Y., Hu, J., Wang, R., Zhao, L., Wang, Y., & Kai, G. (2020). First report of corm rot on saffron caused by Penicillium solitum in China. Plant Disease, 104(2), 579–579.
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Shahnaz, E., Banday, S., Dar, Z. A., Lone, A. A., Habib, M., Nisa, S. U., Kumar, A., Iqbal, S., & Jhang, T. (2024). New and emerging trends in phytopathology of medicinally bioactive geographical indicator of Kashmir: Saffron (Crocus sativus L.). Journal of Medicinal and Aromatic Plant Sciences, 46(1), 10–16. https://doi.org/10.62029/jmaps.v46i1.shahnaz