Journal of Medicinal and Aromatic Plant Sciences

Volume: 40 Issue: 4

  • subscription
  • New Variety Release

Registration of ‘CIM-Pushti’: Withanolide-A rich, leaf blight tolerant high yielding variety of ashwagandha (Withania somnifera) with good root textural quality

TRIPTA JHANG*, POOJA SINGH, NAUSHAD AHMED, SHIVANI SHUKLA, POOJA MAURYA, PUSHPESH JOSHI, MM GUPTA, KP SHASTRY, SANJAY KUMAR, VKS TOMAR, ABDUL SAMAD, RAKESH PANDEY, AK GUPTA, LN MISHRA, RK LAL

Division of Genetics and Plant Breeding, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O CIMAP, Lucknow, UP-226015,India
*Corresponding Author; Email: [email protected]

Year: 2018, Page: 58-66, Doi: https://doi.org/10.62029/jmaps.v40i4.Jhang

Received: Feb. 22, 2018 Accepted: June 20, 2018 Published: Dec. 31, 2018

Abstract

Ashwagandha (Withania somnifera) is an Indian origin, dryland medicinal plant sought across the world as a potent rejuvenative adaptogen owing to its various pharmacological properties. The roots are the most frequently used plant part of Ashwagandha since ancient India Vedic era, as the tissue is enriched with Withanolide A that imparts neuroprotection, mitigates stress and helps to increase brain health. Under a Withanolide targeted breeding program at CSIRCIMAP, a new variety ‘CIM-Pushti’ has been developed through intraspecific hybridization between ‘Nagouri’ X ‘Kashmiri’, the two major ecotypes prevalent in India. The new variety combines the root quality of Nagouri type (cultivated and annual type) and biomass yield of Kashmiri type (wild, perennial type) in one genotype background. The characteristics of the new variety includes highly wavy, subcoriaceous, light green leaves with semi erect, strong culm and yellow orange mature berries. Roots are whitish cream with nonseparable rind, fine pulverisable, brittle roots with high starch to fibre ratio and Withanolide-A content averaging 0.713mg/g dry weight basis. It is also high in Withanolide- B(0.460mg/g dry weight basis) and negligible presence of Withanone. It matures in 168-178 days producing average yield of 9-10 quintal roots per hectare. It is also tolerant to leaf blight caused by Alternaria alternata. The variety is suitable for cultivation in the Indian agro-climatic zones (VIII,XIII & XIV) i.e. Central Plateau, Western dry region and Gujarat planes regions of India.

Keywords: Alternaria alternate, Nagouri Ashwagandha, Withania somnifera, Withanolide A

References

Abraham A, Kirson I, Glotter E, Lavie D. 1968. A chemotaxonomic study of Withania somnifera L. Dunal. Phytochem 7: 957–962.

Ahmad H, Khandelwal K, Samuel SS, Tripathi S, Mitra K, Sangwan RS, Shukla R, Dwivedi AK. 2016. Neuro-protective potential of a vesicular system of a standardized extract of a new chemotype of Withania somnifera Dunal (NIMTLI 118RT+) against cerebral stroke in rats. Drug Deliv 23: 2630-2641.

Ahmed N, Singh P and Jhang T. 2017. Evaluation of selected lines of Withania somnifera for their root quality and yield performance in Inter Drought V International Conference held during February 21-25,2017 at Hyderabad India organized by ICRISAT, Patencharu, India (IDT7-41).

Akhoon BA, Pandey S, Tiwari S, Pandey R. 2016. Withanolide A offers neuroprotection, ameliorates stress resistance and prolongs the life expectancy of Caenorrhabditis elegans. Exp Gerontol 78:47-56.

Arpan, Ankesh, Karuna Shanker, Gupta AK, Jhang T. 2014. RNA-Seq Analysis of Two Elite Lines of Withania somnifera to Evaluate Potential of Heterosis. Indian J Genet Pl Breed 74: 612-619.

Arpan, Gupta AK, Jhang T. 2015 Prediction of Withanolide Heterosis using RNA Seq in Withania somnifera at Vth International conference Next generation Genomics and Integrated breeding for crop Improvement 18- 20 Feb 2015, ICRISAT,Patencharu India.

Arpan, Santoshi Vinita, Shankar K, Gupta AK, Tripta Jhang 2014. Heterosis in Withania somnifera hybrids at National Convention and Seminar on Leveraging Aromatic and Medicinal Plants and Products, CSIR-CIMAP, Lucknow. 8-9 March, 2014. Medicinal and Aromatic Plants, Society of India, Lucknow. p6.

Atal CK, Gupta OP, Ragunathan K, & Dhar KL. 1975. Pharmacognosy and phytochemistry of Withania somnifera (Linn.) Dunal. New Delhi, Central Council of Research in Indian Medicine and Homeopathy pp. 6–18.

Chaurasiya ND, Uniyal GC, Payare Lal, Misra L, Sangwan NS, Tuli R, Sangwan RS. 2008. Analysis of withanolides in root and leaf of Withania somnifera by HPLC with photodiode array and evaporative light scattering detection. Phytochem Anal 19: 148-154.

Dar NJ, Satti NK, Dutt P, Hamid A, Ahmad M. 2017. Attenuation of glutamate-induced excitotoxicity by Withanolide A in neuron like cell: Role for P13K/Akt/MAPK signalling pathway. Mol Neurobiol doi: 10.1007/s12035- 017-0515-5.

Dhar RS, VermaV, Suri K A, Sangwan R S, Satti N K, Kumar A, Tuli R, Qazi G N . 2006. Phytochemical and genetic analysis in selected chemotypes of Withania somnifera. Phytochem 67: 2269-2276.

Jhang T, Singh P, Ahmed N. 2017. Allele specific expression regulatory divergence between Nagouri and Kashmiri Ecotypes of Ashwagandha (Withania somnifera) and their hybrids during the Hargovind Khorana Memorial Sympoisum on Genes, Genome and Membrane biology held from 03-05 Dec 2017 at NABI, Chandigarh India.

Jhang T, Singh P, Ahmed N, Shastry KP, Sanjay Kumar. 2017. Development of metabolite targeted plant variety in Withania somnifera to meet Pharmaceutical and Nutraceutical Demand(WSE 10193 at IISF, Women Scientist and Entrepreneur’s Conclave: Game Changers Driving Science for New India, Anna University, Chennai15- 16 October, 2017.

Jhang T, Shatry KP, Mishra HO Gupta AK Lal RK and Sangwan NS. 2015. Ashwagandha the rejuvenating Drug, A boon for rainfed agriculture. India International Science Festival.5-7 Dec, 2015. IIT Delhi .

Jhang T. 2015. Characterization of core collections: first step for precision breeding for metabolite &ideotype in Withania somnifera at CSIR-CIMAP International Conference on Medicinal plants: Resources for affordable new generation Healthcare Mar 20-22, 2015 at CSIR-CIMAP, Lucknow, India

Patnaik R, Kumar G. 2016.Exploirnig neuroprotective potential of Withania somnifera phytochemicals by inhibition of GluN2B containing NMDA receptors: An in silico study. Medical Hypothesis 92: 35-43.

Rayees S, Malik F. 2017.Withania somnifera: From traditional use to evidence based medicinal prominence In Science of Ashwagandha: Preventive and Therapeutic Potentials Ed Wadhwa R, Kaul SC, 2017, 81- 103. Springer.

Sangwan RS, Chaurasiya ND, Lal P, Misra L, Tuli R, Sangwan NS. 2008 .Withanolide-A is inherently de novo biosynthesized in roots of the medicinal plant Ashwagandha (Withania somnifera). Physiol Pl 133: 278– 287.

Singh S, Kumar S. 1998. Withania somnifera: The Indian Ginseng Ashwagandha; Central Institute of Medicinal and Aromatic Plants: Lucknow, India, 1998.

Singh V, Singh B, Sharma A, Kaur K, Gupta AP, Salar RK, Hallan V, Pati PK.2017. Leaf spot disease adversely affects human health promoting constituents and withanolide biosynthesis in W. somnifera(L) Dunal. J Appl Microbiol 122:153-165.

Srivastava P, Tiwari N, Yadav AK, Kumar V, Shanker K, Verma RK, Gupta MM, Gupta AK, Khanuja SPS. 2008. Simultaneous quantification of withanolides in W. somnifera by a validated high performance thin layer chromatography method. JAOAC Int 91:1154-1161. 22. http://www.spins.com/retail-measurementservices
 

Cite this article

Tripta Jhang, Pooja Singh, Naushad Ahmed, Shivani Shukla, Pooja Maurya, Pushpesh Joshi, MM Gupta, KP Shastry, Sanjay Kumar, VKS Tomar, Abdul Samad, Rakesh Pandey, AK Gupta, LN Mishra, RK Lal. 2018. Registration of ‘CIM-Pushti’: Withanolide-A rich, leaf blight tolerant high yielding variety of ashwagandha (Withania somnifera) with good root textural quality. J Med Aromat Plant Sci 40: 58-66.
 

Views
579
Downloads
1
Citations