Volume: 36 Issue: 1
Pharmacognostic standerdization, HPTLC and in-vitro antioxidant studies of Averrhoa carambola L. leaves
Year: 2014, Page: 16-33, Doi: https://doi.org/10.62029/jmaps.v36i1.Himanshi
Received: Feb. 12, 2014 Accepted: April 17, 2014 Published: July 1, 2014
This paper deals with the detailed pharmacognostical evaluation, HPTLC studies and in-vitro anti- oxidant activity of the leaves of A. carambola (Oxalidacea). The physicochemical, morphological and histological parameters studied in this study may be proposed as parameter to establish the authenticity of A. carambola. The microscopical evaluation defines the presence of anomocytic stomata , multicellular trichomes, calcium oxalate crystals, starch grains, spiral vessels and characterstic collenchymas and vascular bundles. Various leaf constants were obtained. In physico-chemical evaluation total ash, acid- insoluble ash, water soluble ash and extractive value using different solvent were studied. Fluorescence analysis and elemental ash analysis was attempted to identify fluorescence and various elements such as iron, calcium, sulphates, nitrates, carbonates, etc. in Averrhoa carambola leaves.
Successive solvent extracts and crude extracts including ethanolic, hydro –alsoholic and water extract, were subjected to preliminary chemical test revealing the presence of carbohydrates, alkaloids, saponins, tannins, and cardiac glycosides. HPTLC studies were used to quantify gallic acid and quercetin content of A. carambola leaves. The concentration of gallic acid and quercetin in test sample was found to be 0.271 mg /g and 0.784 mg/g respectively. Anti- oxidant activity was carried out through model viz. DPPH assay and hydrogen peroxide assay and results were found positive.
Keywords: Anti-oxidant activity, Averrhoa carambola, Gallic acid, HPTLC, Quercetin
Ahmad H, Mishra A, Gupta R, Saraf SA. 2011.Determination of gallic acid in Michelia champaca L. (Champa) leaves and stem bark by HPTLC. Der Pharmacia Lettre 3: 307-317.
Anon. The Ayurvedic Pharmacopoeia of India, Govt. of India, Ministry of Health and Family Welfare Dept, AYUSH, P 230.
Anon. 1991. Guidelines for the Assessment of Herbal Medicines, Document No.WHO/TRM/91,4, World Health Organization, Geneva, P 22- 33.
Anon. 1996. Indian Pharmacopoeia, The Controller of Publication, New Delhi, Vol 2, PA-47.
Gupta AK, Tondon N, Sharma M. 2005. Quality Standards Of Indian Medicinal Plants, Indian Council Of Medical Research, New Delhi, Vol 2, P 44.
Harborne JB 2005. Phytochemical Methods.3rd ed, Chapman and Hall, London, P 49-244.
Hussain I, Ullah R, Khurram M, Ullah N. 2011.Phytocemical analysis of selected medicinal plant. Afr J Biotechnol 10: 7487-7492.
Khandelwal KR. 2006. Practical Pharmacognosy- Techniques and Experiments.Nirali Prakashan, Pune, India, P 132- 161.
Moresco HH, Queiroz GS, Pizzolatti GM. 2012. Chemical constituents and evaluation of the toxic and antioxidant activities of Averrhoa Carambola Leaves. Rev Bras Farmacogn 22: 319-324.
Prabhu K, Karar PK, Hemlatha S, Ponnuduraik. 2011. A preliminary chromatographic detection of phenolic compounds from ethanolic stem extracts of Viburnum Linn. Species by TLC and PC. Der Pharmacia Sinica 2: 74-80.
Singh R, Lawania RD, Mishra A, Gupta R.2010. Role of Cordia Dichotoma seeds and leaves extract in degenerative disorders. Int J Pharm Sci Rev Res 2: 21-24.
Tang LK, Ling LPK, Koh RY, Chye SM.2012. Screening of anti-dengue activity in methanolic extracts of medicinal plants. Complement Altern Med 12: 3.
Wagner H, Blat S, Zgainski EM .1984. Plant Drug Analysis, Springer-Verlag, Berlin Heidelberg, New York, P 93-94.
Wagner H, Blat S, Zgainski EM .1996. Plant Drug Analysis, Springer-Verlag, Berlin Heidelberg, New York, P 196.
Wagner H, Blat S, Zgainski EM .1996. Plant Drug Analysis, Springer-Verlag, Berlin Heidelberg, New York, P 306.
Wetwitayaklung P, Charoenteeraboon J, Limmatvapirat C. 2012. Antioxidant activities of some Thai and exotic fruits cultivated in Thailand. Res J Pharm Biol Chem Sci 3: 12-21.
© Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow (India)
Himanshi, Mridul Mayank, Avinash Shukla, Anurag Mishra. 2014. Pharmacognostic standerdization, HPTLC and in-vitro antioxidant studies of Averrhoa carambola L. leaves. J Med Aromat Plant Sci 36: 16-33.
