Volume: 38 Issue: 2
Effect of seed priming with plant growth regulators and temperature on Withania somnifera L. DUNAL
Year: 2016, Page: 56-61, Doi: https://doi.org/10.62029/jmaps.v38i2.Kumar
Received: Nov. 1, 2013 Accepted: Jan. 1, 2015 Published: Dec. 31, 2016
Seeds of Indian ginseng (Withania somnifera var. Poshita and Jawahar-20) were exposed to various pre-soaking treatments (24,48 and 72 hours) with different concentrations of three growth regulators (GA3, IBA, and IAA) ranges from 0.25 to 150 ppm at different temperatures (22, 25 and 280C) to observe the combined effect of these factors on germination. Exposure at 280C with 100ppm GA3 for 24 hours soaking time and 125 ppm GA3 with 72 hours soaking time showed most synergistic effect on germination in J20 (76.66%) and Poshita (73.33%), respectively. Though IBA and IAA also showed significant increase in germination in both the varieties but it was not as effective as GA3. Study concluded that growth regulators and appropriate temperatures play a synergistic role in breaking the seed dormancy by influencing various metabolic activities at this moment.
Keywords: Seed germination, Temperature, Withania somnifera, Plant growth regulators
Arteca RN. 1996. Plant growth substances: principles and applications. Chapter 3: Chemistry, biological effects and mechanism of action. Chapman & Hall 115 Fifty Avenue New York, NY 10003, pp. 66.
Bakrim A, Lamhamdi M, Sayah F, Chibi F. 2007. Effects of plant hormones and 20-hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedling growth. Afr J Biotech 6: 2792-2802.
Bao J, Zhang S. 2011. Changes in endogenous hormone contents of pear stock (Pyrus betulaefolia Bge. and Pyrus calleryana Dcne.) seeds during cold stratification. Afr J Biotech 10: 16813-16825.
Bhardwaj RK, Bhardwaj A, Gangwar SK.2012. Efficacy of ashwagandha (Withania somnifera) supplementation on haematological and immunological parameters of japanese quails. Inter J Sci Nat 3: 476-478.
Ellis RH, Barret S. 1994. Alternating temperatures and rate of seed germination in lentil. Ann Bot 74: 519-524.
Ganai KA, Nawchoo IA. 2002. In vitro seed germination studies on Arnebia benthamii. Ind J Pl Physiol 7: 252-255.
Gehan GM, Mona FAA. 2011. Effect of gibberellic acid and indole 3-acetic acid on improving growth and accumulation of phytochemical composition in Balanitesa egyptiaca plants. Amer J Plant Physiol 6: 36-43.
Giri D, Tamta S. 2012. Effect of pre-sowing treatments on seed germination in Hedychium spicatum: An important vulnerable medicinal plant of Indian Himalayan region. Sci Res Essays 7: 1835-1839
Kambizi L, Adebola PO, Afolayan AJ. 2006. Effects of temperature, pre- chilling and light on germination of Withania somnifera a high value medicinal plant. South Afr J Bot 72: 11-14.
Khanna PK, Kumar A, Chandra R, Verma V.2013. Germination behaviour of seeds of Withania somnifera (L.) Dunal: a high value medicinal plant. Physiol Mol Biol Pl 19: 449–454
Kumar N, Shankhdhar SC, Shankhdhar D.2011. Effect of phytohormones pretreatment on physiology of seed germination in Withania somnifera. J Indian Bot Soc 91: 384-386.
Mohan KK, Reddy AR, Sharma S, Jyotsna B.2012. Effect of physical and chemical treatments on dormancy breaking, germination and vigour of certain medicinal plants. J Pharmacognosy 3: 71-72.
Peters J. 2000. Tetrazolium Testing Handbook, Contribution No. 29. The Handbook on Seed Testing. Prepared by the Tetrazolium Subcommittee of the Association of Official Seed Analysts. Part 2. Lincoln, Nebraska.
Saritha KV, Naidu CV. 2007. In vitro flowering of Withania somnifera Dunal. an important antitumor medicinal plant. Pl Sci 172: 847–851.
Shen H, Zhu L, Bu QY, Huq E. 2012. Max2 affects multiple hormones to promote photomorphogenesis. Mol Pl 5: 224-236.
Subhas I, Sachin B. 2012. Comparative study of seed germination and percentage of fungal infection of Ashwagandha (Withania somnifera (L.) Dunal.). Res J Recent Sci 1: 80-82.
Vakeswara, V, Krishnasamy V. 2003. Influence of plant growth regulators in germination of Withenia sominifera Dunal seeds. Seed Tech 25: 207.
Yarnia M, Tabrizi EFM. 2012. Effect of seed priming with different concentrations of GA3, IAA and kinetin on Azarshahr onion germination and seedling growth. J Basic Appl Sci Res 2: 2657-2661.
Yucel E, Yilmaz G. 2009. Effects of different alkaline metal salts (NaCl, KNO3), acid concentrations (H2SO4) and growth regulator (GA3) on the germination of Salvia cyanescens Boiss. & Bal. seeds. Guj Sci 3: 123-127.
© Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow (India)
Narendra Kumar, SC Shankhdhar, Deepti Shankhdhar. 2016. Effect of seed priming with plant growth regulators and temperature on Withania somnifera L. DUNAL. J Med Aromat Plant Sci 38: 56-61.