Volume: 42 Issue: 1
CIM-Sushil: A high vindoline yielding variety of Catharanthus roseus (L.) G. Don
Year: 2020, Page: 51-63, Doi: https://doi.org/10.62029/jmaps.v42i1.Gupta
Received: Feb. 12, 2020 Accepted: March 16, 2020 Published: July 1, 2020
Catharanthus roseus produces many terpenoid indole alkaloids (TIAs) but is famous for its scarce and costly anticancer bisindole alkaloids, vinblastine and vincristine that are formed by coupling of monomeric TIAs, catharanthine and vindoline. Biosynthetic potential of the plant for bisindoles is very low due to their high cytotoxicity. The best option for industrial production of bisindoles is their semisynthesis from the monomers. For this, catharanthine is sourced easily as it is produced throughout the plant and also in cell suspensions. But, sourcing vindoline is the main bottleneck since its biosynthesis is limited to green leaves and requires high level of cellular differentiation, which prevents its bioreactor-based production through cell culture. Vindoline synthesis is non-economical and its production in heterologous systems is still in infancy requiring much optimization. Thus, the plant remains the only major source of vindoline. Improving vindoline availability would therefore significantly reduce the exorbitant cost of bisindoles. Here, we report CIM-Sushil, a new high vindoline yielding C. roseus variety developed through EMS-induced mutation breeding approach. It has dwarf character, spreading/bushy growth (wide canopy) and small dark green leaves. Its leaves have ~0.2% vindoline content and ~5% total alkaloid content (% dry weight basis). Its estimated dry leaf yield potential is ~2418.3 kg/ha, estimated vindoline yield potential is ~4.8 kg/ha and estimated total alkaloid yield potential is ~120.9 kg/ha. It outperformed the check varieties, Nirmal and Dhawal, in content as well as yield of vindoline and total alkaloid. Its vindoline content is comparable to the global benchmark. Additionally, CIM-Sushil will be greatly beneficial for research purpose.
Keywords: Dwarf Character, Multilocation Trial, Mutation Breeding, Plant Variety, Vindoline
Chung I-M, Kim E-H, Li M, Peebles CAM, Jung WS, Song H-K, Ahn J-K, San K-Y. 2011. Screening 64 cultivars Catharanthus roseus for the production of vindoline, catharanthine, and serpentine. Biotechnol Prog 27: 937-943.
De Luca V, Cutler AJ. 1987. Subcellular localization of enzymes involved in indole alkaloid biosynthesis in Catharanthus roseus. Plant Physiol 85: 1099-1102.
Dwivedi S, Singh M, Singh AP, Singh V, Uniyal GC, Khanuja SPS, Kumar S. 2001. Registration of a new variety Prabal of Catharanthus roseus. J Med Arom Pl Sci 23: 104-106.
Goraya GS, Ved DK. 2017. Medicinal Plants in India: An assessment of their demand and supply. National Medicinal Plants Board, Ministry of AYUSH, Government of India, New Delhi and Indian Council of Forestry Research and Education, Dehradun.
Khanuja SPS, Shasany AK, Darokar MP, Kumar S. 1999. Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils. Plant Mol Biol Rep 17: 74.
Kulkarni RN, Baskaran K, Chandrashekara RS, Kumar S. 1999. Inheritance of morphological traits of periwinkle mutants with modified contents and yields of leaf and root alkaloids. Plant Breed 118: 71-74.
Kulkarni RN, Baskaran K, Chandrashekara RSR, Khanuja SPS, Darokar MP, Shasany AK, Uniyal GC, Gupta MM, Kumar S. 2003. ‘Dhawal‘, a high alkaloid producing periwinkle plant. U. S. Patent No. 6,548,746 B1.
Mall M. 2017. Molecular studies related to key terpenoid indole alkaloid biosynthesis in Catharanthus roseus. Ph.D. thesis, Jawaharlal Nehru University, New Delhi, India.
Mall M, Singh P, Kumar R, Shanker K, Gupta AK, Khare P, Shasany AK, Khatoon S, Sundaresan V, Baskaran K, Yadav S, Shukla AK. 2020. Phenotypic, genetic and expression profiling of a vindoline-rich genotype of Catharanthus roseus. (Under Communication).
Mall M, Verma RK, Gupta MM, Shasany AK, Khanuja SPS, Shukla AK. 2019. Influence of seasonal and ontogenic parameters on the pattern of key terpenoid indole alkaloids biosynthesized in the leaves of Catharanthus roseus. S Afr J Bot 123: 98-104.
Merck website. 2019. https:// www.sigmaaldrich.com/
Mishra P, Singh M, Dwivedi S, Kumar S. 2000. Descriptors of periwinkle Catharanthus roseus. J Med Arom Pl Sci 22: 268-272.
Onofri A, Pannacci E. 2014. Spreadsheet tools for biometry classes in crop science programmes. Communications in Biometry and Crop Science 9: 43-53.
Pandey SS, Singh S, Vivek Babu CS, Shanker K, Srivastava NK, Shukla AK, Kalra A. 2016. Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis. Sci Rep 6: 26583.
Qu Y, Easson MLAE, Froese J, Simionescu R, Hudlicky T, De Luca V. 2015. Completion of the seven-step pathway from tabersonine to the anticancer drug precursor vindoline and its assembly in yeast. Proc Natl Acad Sci USA 112: 6224-6229.
Qu Y, Thamm AMK, Czerwinski M, Masada S, Kim KH, Jones G, Liang P, De Luca V. 2018. Geissoschizine synthase controls flux in the formation of monoterpenoid indole alkaloids in a Catharanthus roseus mutant. Planta 247: 625-634.
Rai SP, Luthra R, Kumar S. 2003. Salt-tolerant mutants in glycophytic salinity response (GSR) genes in Catharanthus roseus. Theor Appl Genet 106: 221–230.
Rajeswara Rao BR, Syamasundar KV, Rajput DK, Nagaraju G, Adinarayana G. 2012. Potential species of medicinal plants for cultivation in Deccan region. J Pharmacognosy 3: 96-100.
Ravikumar K, Noorunnisa Begum S, Ved DK, Bhatt JR, Goraya GS. 2018. Compendium of traded Indian medicinal plants. Foundation for Revitalization of Local Health Traditions (FRLHT), Bengaluru, India.
Roepke J, Salim V, Wu M, Thamm AMK, Murata J, Ploss K, Boland W, De Luca V. 2010. Vinca drug components accumulate exclusively in leaf exudates of Madagascar periwinkle. Proc Natl Acad Sci USA 107: 15287–15292.
Shasany AK, Darokar MP, Dhawan S, Gupta AK, Gupta S, Shukla AK, Patra NK, Khanuja SPS. 2005. Use of RAPD and AFLP markers to identify inter- and intraspecific hybrids of Mentha. J Hered 96: 542-549.
Shukla AK, Khanuja SPS. 2013. Catharanthus roseus: The metabolome that represents a unique reservoir of medicinally important alkaloids under precise genomic regulation. In: Barh D, ed. OMICS Applications in Crop Science. Boca Raton: CRC Press (Taylor & Francis Group), 325–384.
Shukla YN, Rani A, Kumar S. 1997. Effect of temperature and pH on the extraction of total alkaloids from Catharanthus roseus leaves. J Med Arom Pl Sci 19: 430-431.
Tiwari R, Awasthi A, Mall M, Shukla AK, Satya Srinivas KVN, Syamasundar KV, Kalra A. 2013. Bacterial endophyte-mediated enhancement of in planta content of key terpenoid indole alkaloids and growth parameters of Catharanthus roseus. Ind Crops Prod 43: 306– 310.
© CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015
Anil K Gupta, Ashutosh K Shukla, Pooja Singh, Maneesha Mall, Seema Yadav, Ajit K Shasany, Karuna Shanker, K Baskaran, Velusamy Sundaresan, Mohammed Talha, Abhilasha Srivastava, Soni Gupta, Puja Khare, Daya N Mani, Abdul Samad. 2020. CIM-Sushil: A high vindoline yielding variety of Catharanthus roseus (L.) G. Don. J Med Aromat Plant Sci 42: 51-63.
