Volume: 42 Issue: 4
Chemoprofiling of Artocarpus camansi Blanco. and Artocarpus lakoocha Wall. ex. Roxb. and its antidiabetic potential for modulation of digestive enzymes, protein glycation and glucose uptake in L6 myotubes
Year: 2020, Page: 241-249, Doi: https://doi.org/10.62029/jmaps.v42i4.Sasikumar
Received: Nov. 19, 2020 Accepted: Nov. 27, 2020 Published: Dec. 31, 2020
Acetone extract of the stem bark of Artocarpus camansi and Artocarpus lakoocha afforded 14 compounds. Herein, we report the compounds 3, 8, 9, 10, 11, 12 and 13 for the first time from these species. The structures were elucidated on the basis of various spectroscopic analyses including IR, 1D and 2D NMR, and HRMS data. All the isolated compounds were tested for α-glucosidase, α-amylase and glycation inhibitory activity. Oxyresveratrol displayed prominent IC50 value of 37.38 ± 1.11 µM in -glucosidase inhibitory assay. Compound 3, 4 and 8-13 exhibited moderate -glucosidase inhibitory activity. The effect of glucose uptake performed by 2-NBDG in L6 muscle cells using fluorescent microscope and flow cytometry. The compounds E-resveratrol (1), artobiloxanthone (11) and artonol B. (13) showed glucose uptake of 32.2 %, 32.9 % and 33.5 % respectively.
Keywords: Artocarpus camansi, Artocarpus lakoocha, Antidiabetic, MTT assay, 2-NBDG uptake
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© CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015
P Sasikumar, M Aswathy, K Lekshmy, TR Reshmitha, P Sharathna, Sanjay S Varma, MT Meenu, VV Sivan, P Nisha, P Jayamurthy, KV Radhakrishnan. 2020. Chemoprofiling of Artocarpus camansi Blanco. and Artocarpus lakoocha Wall. ex. Roxb. and its antidiabetic potential for modulation of digestive enzymes, protein glycation and glucose uptake in L6 myotubes. J Med Aromat Plant Sci 42: 241-249.
