Volume: 35 Issue: 3
A simple and reliable method for the determination of tetralone-4-O- ß -D-glucopyranoside and 4-hydroxy- α -tetralone in three species of Ammannia by reversed-phase HPLC
Year: 2013, Page: 136-141, Doi: https://doi.org/10.62029/jmaps.v35i3.Upadhyay
Received: Oct. 5, 2013 Accepted: Dec. 12, 2013 Published: Dec. 31, 2013
The species of genus Ammannia have proven medicinal efficacies, being frequently used in traditional Chinese and Indian systems of medicine. The compounds 4-hydroxy-α-tetralone and its glucoside have been identified as the major bioactive constituents in Ammannia species. A rapid, reliable and reproducible reversed-phase HPLC method was developed for the determination of tetralone-4-O-ß-D-glucopyranoside (1) and 4-hydroxy- α - tetralone (2) in three species of Ammannia namely, A. coccinea, A. verticillata and A. tenuis is repoted here. The chromatographic separation of compounds was performed on a Waters Spherisorb ODS2 (250 X 4.6 mm i.d., 10μm) column by one step gradient elution with 0.1% trifluoroacetic acid (TFA) in water and methanol at a flow rate of 0.4 mL/min, a column temperature at 25±20C and ultra-violet (UV) detection at λ 254 nm. The limit of detection (LOD) and limit of quantification (LOQ) were 0.30 and 0.47 μg/mL for analyte 1 and 1.01 and 1.33 μg/mL for analyte 2, respectively. Satisfactory results with respect to linearity (r2 > 0.999), repeatability (relative standard deviation, RSDd<1.23%) and recovery (96.8-100.8%) were obtained with this method. The developed method was successfully applied for the quantification of these two analytes (1 & 2) in seven different samples of the investigated three Ammannia species.
Keywords: Ammannia species, Tetralone-4-O- ß –Dglucopyranoside, 4-hydroxy- α –tetralone, RP-HPLC
An TY, Hu LH, Chen RM, Chen ZL, Li J, Shen Q. 2003. Anti-diabetes Agents-I: Tetralone derivatives from Juglans regia. Chinese Chem Lett 14: 489-490.
Calixto JB. 2000. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (Phytotherapeutic agents). Brazilian J Med Biol Res 33: 179-189.
Datta A, Srivastava PS. 1997. Variation invinblastine production by Catharanthus roseus , during in vivo and in vitro differentiation. Phytochem 46: 135–137.
Fournet A, Angelo AB, Munoz V, Hocquemiller R, Roblot F, Cave A. 1994. Anti leishmanial activity of a tetralone isolated from Ampelocera edentula, a Bolivian plant used as a treatment for cutaneous leishmaniasis. Planta Med 60: 8-12.
Fujii Y, Fujii H, Yamazaki M. 1983. Separation and determination of cardiac glycosides in Digitalis purpurea leaves by micro high performance liquid chromatography. J Chromatogr 258: 147–153.
International Conference of Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human use. Harmonized tripartite guidelines, validation of analytical procedures : Text and Methodology, Q2 (R1) ,2005. Accessed December 06,2013.(http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guideli nes/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf).
Liang Q, Qu J, Luo G, Wang Y. 2006. Rapid and reliable determination of illegal adulterant in herbal medicines and dietary supplements by LC/MS/MS. J Pharm Biomed Anal 40: 305–311.
Lin WY, Peng CF, Tsai IL, Chen JJ, Cheng MJ, Chen IS. 2005. Anti tubercular constituents from the roots of Engelhardia roxburghiana. Planta Med 71: 171-175.
Lopez- Feria S, Cardenas S, Garcýa-Mesa JA, Valcarcel M. 2008. Classification of extra virgin olive oils according to the protected designation of origin, olive variety and geographical origin. Talanta 75: 937–943.
Mander L, Liu HW. 2010. Comprehensive Natural Products II: Chemistry and Biology. Elsevier, Amsterdam, The Netherlands.
Newman DJ, Cragg GM. 2007. Natural products as sources of new drugs over the last 25 Years. J Nat Prod 70: 461–477.
Shin Y, Liu RH, Nock JF, Holliday D, Watkins CB. 2007. Temperature and relative humidity effects on quality, total ascorbic acid, phenolics and ûavonoid concentrations, and antioxidant activity of strawberry. Postharvest Biol Tech 45: 349–357.
Tistaert C, Dejaegher B, Heyden YV. 2011. Chromatographic separation techniques and data handling methods for herbal fingerprints: A review. Analytica Chemica Acta 690: 148- 161.
Upadhyay HC, Dwivedi GR, Darokar MP, Chaturvedi V, Srivastava SK. 2012. Bioenhancing and anti-mycobacterial agents from Ammannia multiflora. Planta Med 78: 79-81.
Upadhyay HC, Thakur JP, Saikia D, Srivastava SK. 2013. Anti- tubercular agents from Ammannia baccifera (Linn.). Med Chem Res 22: 16-21.
Upadhyay HC, Verma RK, Srivastava SK. 2012. A validated LC method for separation and determination of tetralone-4-O-ß-Dglucopyranoside and 4-hydroxy-α-tetralone in Ammannia multiflora. Chrom Res International, DOI:10.1155/2012/162302.
Upadhyay HC, Verma RK, Srivastava SK.2013. Quantitative determination of bioactive 4-hydroxy-α-tetralone, tetralone-4-O-ß-D-glucopyranoside and ellagic acid in Ammannia baccifera (Linn.) by reversedphase high performance liquid chromatography. J Chrom Sci 51: 21-25.
© Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow (India)
Harish C Upadhyay, Ram K Verma, Santosh K Srivastava. 2013. A simple and reliable method for the determination of tetralone-4-O- ß -D-glucopyranoside and 4-hydroxy- α -tetralone in three species of Ammannia by reversed-phase HPLC. J Med Aromat Plant Sci 35: 136-141.
